PSI Workshop 2023

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Workshop

The PSI Dynamo workshop will take place from August the 16th to August the 18th in the Paul Scherrer Institute.

The workshop provides hands-on training in all practical aspects of tomography and sub-tomogram averaging using our software Dynamo, encompassing all stages of the pipeline: alignment and reconstruction of tilt series, tomogram visualization and archiving, particle selection and extraction, and subtomogram alignment, averaging, refinement and classification. No previous experience is required. Computing infrastructure will be provided in place, and we welcome participants to bring their own data sets to work on them during this four-day course.

Computing resources

Access

We will publish here a brief guide describing the steps to follow to access the computing resources.

Time Schedule

Day 1: Morning
Module Time Subject
0 09:00 - 10:00 Research Talk
1 10:00 - 11:00 General introduction: Hands on
2 11:00 - 12:30 Starters Guide: subtomogram averaging
3 13:30 - 14:00 General modelling
3a 14:00 - 14:30 Models: Filaments
3b 14:30 - 14:45 Models: Vesicles
3c 14:45 - 15:00 Models: Surfaces


Day 1: Afternoon
Module Time Subject
4 Example Template Matching
5 Example Principal Component Analysis
6a Example Alignment of tilt series: Manual
6b Example Alignment of tilt series: GUI
6c Example Alignment of tilt series: command line
7 Example Advanced Starters Guide