PSI Workshop 2023
Contents
Workshop
The PSI Dynamo workshop will take place from August the 16th to August the 18th in the Paul Scherrer Institute.
The workshop provides hands-on training in all practical aspects of tomography and sub-tomogram averaging using our software Dynamo, encompassing all stages of the pipeline: alignment and reconstruction of tilt series, tomogram visualization and archiving, particle selection and extraction, and subtomogram alignment, averaging, refinement and classification. No previous experience is required. Computing infrastructure will be provided in place, and we welcome participants to bring their own data sets to work on them during this four-day course.
Computing resources
Laptops
Participants will bring their own laptops to access a PSI server which will host the data. However, each participant should also install in their laptop prior to the workshop:
- (R2023a version), a licence free trial version is available.
- Dynamo : prior to installation make sure you have a compiler. On Linux you probably have one by default. On Mac, Xcode needs to be installed. On Windows, the recommended option is to install it via Matlab:
https://es.mathworks.com/help/matlab/matlab_external/install-mingw-support-package.html
Then,
>> tar -xvf dynamo_temp_1.1.530.tar
Launch the installed package inside MATLAB.
>> run <DYNAMO_ROOT>/dynamo_activate.m
Wait while some packages are compiled. Activate it again.
Access to PSI resources
This guide describes the steps to follow to access the computing resources inside PSI.
Time Schedule
- On the first day we will present the basic tools in the Dynamo environment.
- The second day will be devoted to a hands-on guide on two practical cases:
- Protocols for Subtomogram Averaging of Membrane Proteins in the Dynamo Software Package Navarro PP, Stahlberg H and Castaño-Díez D (2018). Front. Mol. Biosci. 5:82.
- Step-by-step guide to efficient subtomogram averaging of virus-like particles with Dynamo Scaramuzza S, Castaño-Díez D (2021). PLoS biology. 19(8):e3001318.
- On the third day participants are encouraged to make a flash presentation (3 mins, max. slides) describing their past or planned research in tomography. We will study the particular cases of interest of each participant, and identify tools of possible relevance inside the Dynamo environment, designing with the participants viable processing workflows combining Dynamo and other packages.
| Module | Time | Subject |
|---|---|---|
| 0 | 09:00 - 10:00 | Research Talk |
| 1 | 10:00 - 10:45 | General introduction: Hands-on guide to basic tools. |
| 2 | 11:00 - 12:30 | Starters Guide: subtomogram averaging |
| 3 | 13:30 - 14:00 | General modeling |
| 3a | 14:00 - 14:45 | Models: Filaments Additional material: Coordinated extraction from several filaments |
| 3b | 14:45 - 15:30 | Models: Vesicles |
| 3c | 15:30 - 16:30 | Models: Surfaces |
| Module | Time | Subject |
|---|---|---|
| 4 | 09:00 - 10:15 | Template Matching |
| 5 | 10:15 - 11:30 | Principal Component Analysis |
| 6a | 11:30 - 12:00 | Alignment of tilt series: Manual |
| 6b | 12:00 - 12:30 | Alignment of tilt series: GUI |
| 6c | 12:30 - 12:45 | Alignment of tilt series: command line |
| Module | Time | Subject |
|---|---|---|
| FHV | 13:30 - 15:30 | Complete walkthrough on FHV virus |
| HIV1 | 15:45 - 17:00 | High Resolution workflow for HIV1: preprocessing High Resolution workflow for HIV1: Dynamo manipulations |
Additional material:
- Coordinated extraction from several vesicles
- Walkthrough on creation of 3d scenes
- Walkthrough on placement of averages on table positions
Instructors
- Daniel Castano-Diez (CSIC Bilbao, Spain).
- Raffaele Coray (CSIC Bilbao, Spain).
- Alma Vivas-Lago (CSIC Bilbao, Spain).
