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Revision as of 12:16, 11 August 2021
This walkthrough is a step-by-step guide that teaches how to extract subtomograms from filament-like structures using the Dynamo filament models.
Data
We are going to demonstrate the basic ideas and tools on a synthetic dataset. This data is already available in the catalogue manager. It can be accessed by first opening the catalogue manager using the command:
dcm
After the catalogue manager opens, we create the synthetic tomogram that includes tubular looking objects (filaments) in the following way:
The catalogue manager will create a catalogue to archive the created tomogram and also the models that we will create around it.
A new tomogram entry appears in the catalogue manager. Any further annotations (models) to the tomograms will be linked to this entry. Select the entry and open it:
A tomogram viewer opens. To move through the tomogram slices, you can either use the mouse wheel, click and drag the tomogram slice up and down, or move the position control left and right.
Annotation
